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(A, B) Schematic diagram for tissue sampling protocol of human dilated cardiomyopathy patients with healthy and non-responding human patients, and their respective MDH2 levels (responding n = 12, non-responding n = 8). Images rendered by Biorender.com. Image usage is covered by BioRender’s Academic License Terms. (C) In silico analysis of MDH2 expression <t>in</t> <t>non-ischemic</t> vs . ischemic cardiomyopathy patients (GDS651; normal n = 11 each group) based on <t>Gene</t> <t>Expression</t> <t>Omnibus</t> data. (D) Genotype-tissue expression of MDH2 in selected tissues. (E) GWAS-derived disease associations for MDH2, including lipid metabolism, cardiovascular diseases, and congenital heart defects. Data are presented as mean ± SD. (B, C) Two-tailed unpaired Student’s t-tests. The numbers above graphs indicate p-values. MDH2, malate dehydrogenase 2; GWAS, genome-wide association study; IDCM, idiopathic dilated cardiomyopathy; nTPM, transcripts per million.
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(A, B) Schematic diagram for tissue sampling protocol of human dilated cardiomyopathy patients with healthy and non-responding human patients, and their respective MDH2 levels (responding n = 12, non-responding n = 8). Images rendered by Biorender.com. Image usage is covered by BioRender’s Academic License Terms. (C) In silico analysis of MDH2 expression <t>in</t> <t>non-ischemic</t> vs . ischemic cardiomyopathy patients (GDS651; normal n = 11 each group) based on <t>Gene</t> <t>Expression</t> <t>Omnibus</t> data. (D) Genotype-tissue expression of MDH2 in selected tissues. (E) GWAS-derived disease associations for MDH2, including lipid metabolism, cardiovascular diseases, and congenital heart defects. Data are presented as mean ± SD. (B, C) Two-tailed unpaired Student’s t-tests. The numbers above graphs indicate p-values. MDH2, malate dehydrogenase 2; GWAS, genome-wide association study; IDCM, idiopathic dilated cardiomyopathy; nTPM, transcripts per million.
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(A, B) Schematic diagram for tissue sampling protocol of human dilated cardiomyopathy patients with healthy and non-responding human patients, and their respective MDH2 levels (responding n = 12, non-responding n = 8). Images rendered by Biorender.com. Image usage is covered by BioRender’s Academic License Terms. (C) In silico analysis of MDH2 expression <t>in</t> <t>non-ischemic</t> vs . ischemic cardiomyopathy patients (GDS651; normal n = 11 each group) based on <t>Gene</t> <t>Expression</t> <t>Omnibus</t> data. (D) Genotype-tissue expression of MDH2 in selected tissues. (E) GWAS-derived disease associations for MDH2, including lipid metabolism, cardiovascular diseases, and congenital heart defects. Data are presented as mean ± SD. (B, C) Two-tailed unpaired Student’s t-tests. The numbers above graphs indicate p-values. MDH2, malate dehydrogenase 2; GWAS, genome-wide association study; IDCM, idiopathic dilated cardiomyopathy; nTPM, transcripts per million.
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(A, B) Schematic diagram for tissue sampling protocol of human dilated cardiomyopathy patients with healthy and non-responding human patients, and their respective MDH2 levels (responding n = 12, non-responding n = 8). Images rendered by Biorender.com. Image usage is covered by BioRender’s Academic License Terms. (C) In silico analysis of MDH2 expression <t>in</t> <t>non-ischemic</t> vs . ischemic cardiomyopathy patients (GDS651; normal n = 11 each group) based on <t>Gene</t> <t>Expression</t> <t>Omnibus</t> data. (D) Genotype-tissue expression of MDH2 in selected tissues. (E) GWAS-derived disease associations for MDH2, including lipid metabolism, cardiovascular diseases, and congenital heart defects. Data are presented as mean ± SD. (B, C) Two-tailed unpaired Student’s t-tests. The numbers above graphs indicate p-values. MDH2, malate dehydrogenase 2; GWAS, genome-wide association study; IDCM, idiopathic dilated cardiomyopathy; nTPM, transcripts per million.
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(A, B) Schematic diagram for tissue sampling protocol of human dilated cardiomyopathy patients with healthy and non-responding human patients, and their respective MDH2 levels (responding n = 12, non-responding n = 8). Images rendered by Biorender.com. Image usage is covered by BioRender’s Academic License Terms. (C) In silico analysis of MDH2 expression <t>in</t> <t>non-ischemic</t> vs . ischemic cardiomyopathy patients (GDS651; normal n = 11 each group) based on <t>Gene</t> <t>Expression</t> <t>Omnibus</t> data. (D) Genotype-tissue expression of MDH2 in selected tissues. (E) GWAS-derived disease associations for MDH2, including lipid metabolism, cardiovascular diseases, and congenital heart defects. Data are presented as mean ± SD. (B, C) Two-tailed unpaired Student’s t-tests. The numbers above graphs indicate p-values. MDH2, malate dehydrogenase 2; GWAS, genome-wide association study; IDCM, idiopathic dilated cardiomyopathy; nTPM, transcripts per million.
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(A, B) Schematic diagram for tissue sampling protocol of human dilated cardiomyopathy patients with healthy and non-responding human patients, and their respective MDH2 levels (responding n = 12, non-responding n = 8). Images rendered by Biorender.com. Image usage is covered by BioRender’s Academic License Terms. (C) In silico analysis of MDH2 expression in non-ischemic vs . ischemic cardiomyopathy patients (GDS651; normal n = 11 each group) based on Gene Expression Omnibus data. (D) Genotype-tissue expression of MDH2 in selected tissues. (E) GWAS-derived disease associations for MDH2, including lipid metabolism, cardiovascular diseases, and congenital heart defects. Data are presented as mean ± SD. (B, C) Two-tailed unpaired Student’s t-tests. The numbers above graphs indicate p-values. MDH2, malate dehydrogenase 2; GWAS, genome-wide association study; IDCM, idiopathic dilated cardiomyopathy; nTPM, transcripts per million.

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: MDH2 S246 phosphorylation protects mitochondria against hypoxia/reoxygenation injury due to acetate/lactate metabolism

doi: 10.4196/kjpp.25.359

Figure Lengend Snippet: (A, B) Schematic diagram for tissue sampling protocol of human dilated cardiomyopathy patients with healthy and non-responding human patients, and their respective MDH2 levels (responding n = 12, non-responding n = 8). Images rendered by Biorender.com. Image usage is covered by BioRender’s Academic License Terms. (C) In silico analysis of MDH2 expression in non-ischemic vs . ischemic cardiomyopathy patients (GDS651; normal n = 11 each group) based on Gene Expression Omnibus data. (D) Genotype-tissue expression of MDH2 in selected tissues. (E) GWAS-derived disease associations for MDH2, including lipid metabolism, cardiovascular diseases, and congenital heart defects. Data are presented as mean ± SD. (B, C) Two-tailed unpaired Student’s t-tests. The numbers above graphs indicate p-values. MDH2, malate dehydrogenase 2; GWAS, genome-wide association study; IDCM, idiopathic dilated cardiomyopathy; nTPM, transcripts per million.

Article Snippet: The MDH2 mRNA expression profile in ischemic and non-ischemic humans was downloaded from the Genome Expression Omnibus (GEO) database of the National Center for Biotechnology Information (GDS651, http://www.ncbi.nlm.nih.gov/geo/ ).

Techniques: Sampling, In Silico, Expressing, Gene Expression, Derivative Assay, Two Tailed Test, GWAS